Ultraviolet Difference Spectral Studies on Concanavalin A. Carbohydrate Interaction
نویسندگان
چکیده
منابع مشابه
Ultraviolet Difference Spectral Studies on Concanavalin A
The interaction of concanavalin A, the phytohemagglutinin of the jack bean, with a variety of glycosides has been studied by the technique of ultraviolet difference spectroscopy. Whereas methyl a-D-glUC0and manno-pyranoside gave rise to relatively low intensity difference spectra, p-nitrophenyl a-D-mannopyranoside and a-D-glucopyranoside yielded large difference spectra upon interaction with co...
متن کاملCarboxyl Groups in Carbohydrate-concanavalin a Interaction
I. The role of carboxyl groups in the binding of specific carbohydrates to concanavalin A, the phytohemagglutinin of the jack bean, has been studied using two techniques: (I) H + titration in the presence and absence of methyl a-D-mannopyranoside, a specifically bound carbohydrate ligand and (2) chemical modification of the protein carboxyl groups employing glycine methyl ester and a water-solu...
متن کاملProtein-carbohydrate interaction. VII. Physical and chemical studies on concanavalin A, the hemagglutinin of the jack bean.
Concanavalin A yielded a monodiperse pattern in the analytical ultracentrifuge in the pH range 2-5. The sedimentation coefficient, sOZ~,~ in pH 5 acetate buffer containing 0.1 M NaCl (total I’/2 = 0.20) was 4s. At pH 7 and above, a two-peak schlieren pattern was observed, the faster component (about 7s) probably representing a dimer. On the basis of an s~o.~ of 3.9s; a D 20.~ of 5.43 X 10-T cm2...
متن کاملProtein-carbohydrate interaction. IV. Application of the quantitative precipitin method to polysaccharide-concanavalin A interaction.
Concanavalin A, a globulin found in the jack bean, reacts specifically to form a precipitate with a restricted group of branched polysaccharides. The various parameters optimal for this interaction were investigated by the quantitative precipitin method with a dextran as the precipitating polysaccharide. In this manner, an assay for concanavalin A activity was established. The analogy of this i...
متن کاملStudies on the ultraviolet difference spectra of proteins and polypeptides.
Changes in the ultraviolet absorption spectra of proteins associated with dcnaturation or proteolysis have been intensively investigated in recent years. These studies have been, in the main, restricted to the difference spectra of proteins and model compounds in the region between 270 and 300 rnp. The changes in this region have been assigned almost entirely to the alterations in the environme...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1970
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1970.tb01116.x